il2rg cytokine receptor  (5 PRIME)

Journal: Parasites & Vectors

Article Title: Serum biochemical parameters and cytokine profiles associated with natural African trypanosome infections in cattle

doi: 10.1186/s13071-017-2255-9

Figure Lengend Snippet: Comparison of serum cytokines between infected and uninfected cattle in Accra. Levels of a IL-10 b IL-4 c IL-12 d IFN-γ e TNF-α. Dots represent individual cytokine values in cattle. Data from time point 1 at Accra were used in the analyses. The horizontal bar represents the median. Statistical significance was tested using the Mann-Whitney U-test, *P < 0.05, ** P < 0.01

Article Snippet: Serum concentrations of IL-10, IL-4, IFN-γ, TNF-α and IL-12 were measured using commercially available sandwiched bovine cytokine ELISA kits (Cusabio Biotech Co., Ltd., Wuhan, China), following the manufacturers’ protocols.

Techniques: Comparison, Infection, MANN-WHITNEY

Journal: Parasites & Vectors

Article Title: Serum biochemical parameters and cytokine profiles associated with natural African trypanosome infections in cattle

doi: 10.1186/s13071-017-2255-9

Figure Lengend Snippet: Comparison of serum cytokines between infected and uninfected cattle in Adidome. Levels of a IL-10 b IL-4 c IL-12 d IFN-γ e TNF-α. Dots represent individual cytokine values in cattle. Data from time point 4 at Adidome were used in the analyses. The horizontal bar represents the median. Statistical significance was tested using the Mann-Whitney U-test

Article Snippet: Serum concentrations of IL-10, IL-4, IFN-γ, TNF-α and IL-12 were measured using commercially available sandwiched bovine cytokine ELISA kits (Cusabio Biotech Co., Ltd., Wuhan, China), following the manufacturers’ protocols.

Techniques: Comparison, Infection, MANN-WHITNEY

Journal: Parasites & Vectors

Article Title: Serum biochemical parameters and cytokine profiles associated with natural African trypanosome infections in cattle

doi: 10.1186/s13071-017-2255-9

Figure Lengend Snippet: Th1/Th2 cytokine concentration in infected cattle at Adidome and Accra . a IL12/ IL-10. b IL-12/IL-4. c INF-γ/IL-10. d IFN-γ/IL-4. e TNF-α/IL-10. f TNF-α/IL-4. Data from time point 1 at Accra and time point 4 at Adidome were used in the analyses. The horizontal bar represents the median. Statistical significance was tested using the Mann-Whitney U-test, * P < 0.05

Article Snippet: Serum concentrations of IL-10, IL-4, IFN-γ, TNF-α and IL-12 were measured using commercially available sandwiched bovine cytokine ELISA kits (Cusabio Biotech Co., Ltd., Wuhan, China), following the manufacturers’ protocols.

Techniques: Concentration Assay, Infection, MANN-WHITNEY

Journal: Parasites & Vectors

Article Title: Serum biochemical parameters and cytokine profiles associated with natural African trypanosome infections in cattle

doi: 10.1186/s13071-017-2255-9

Figure Lengend Snippet: Cytokine ratios in individual cattle at Adidome and Accra over six time points . a IL-12/ IL-10 levels in two cows (AD-3780 and AD-7759) infected throughout all six time points and a cow (AD-7743) uninfected throughout all the time point at Adidome. b IL-12/IL-4 levels in two cows (AD-3780 and AD-7759) infected throughout all six time points and a cow (AD-7743) uninfected throughout all the time point at Adidome. c IL-12/ IL-10 levels in two cows (UG-164 & UG-166) infected throughout all six time points in Accra. d IL-12/IL-4 levels in two cows (UG-164 & UG-166) infected throughout all six time points in Accra. Time points are at 4 to 5 week intervals

Article Snippet: Serum concentrations of IL-10, IL-4, IFN-γ, TNF-α and IL-12 were measured using commercially available sandwiched bovine cytokine ELISA kits (Cusabio Biotech Co., Ltd., Wuhan, China), following the manufacturers’ protocols.

Techniques: Infection

Journal: PLoS Genetics

Article Title: Murine Leukemias with Retroviral Insertions at Lmo2 Are Predictive of the Leukemias Induced in SCID-X1 Patients Following Retroviral Gene Therapy

doi: 10.1371/journal.pgen.1000491

Figure Lengend Snippet: Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.

Article Snippet: , Il2rg , Cytokine receptor , 5 prime , 6.396 kb , inv.

Techniques: Cloning, Transduction

Journal: PLoS Genetics

Article Title: Murine Leukemias with Retroviral Insertions at Lmo2 Are Predictive of the Leukemias Induced in SCID-X1 Patients Following Retroviral Gene Therapy

doi: 10.1371/journal.pgen.1000491

Figure Lengend Snippet: A) LM-PCR identified two independent insertions in tumor 7107, 5′ of the eight coding exons of the Il2rg gene. The two viral chromosomal junctions were independently cloned and sequenced. The insertions were 396 bp apart and should be present in the same clone if they occurred on the same chromosome. Partial sequence for clone 23-10 shows the region of identity with clone 23-1 (green). For clone 23-1, we found viral sequence 5′ of this region instead of chromosome X genomic sequence. B) Two insertions in the Irs2 gene were also isolated from the same tumor. Primers were designed for one allelic insertion for Irs2 and Il2rg and real time PCR performed on the genomic DNA. Primers for a chromosome X sequence were used as reference and show a copy number of 1 for this male mouse. As shown, each insertion comprises about 50% of total tumor genomic DNA. We ruled out polyploidy for Irs2 and Il2rg genes (see ). C) Panel shows a heat map of gene expression data using the mouse Affymetrix array. The expression of genes near high frequency insertions cloned from 7107 is shown with respect to normal thymus. Log 2 intensity scale is shown under the heat map.

Article Snippet: , Il2rg , Cytokine receptor , 5 prime , 6.396 kb , inv.

Techniques: Clone Assay, Sequencing, Isolation, Real-time Polymerase Chain Reaction, Gene Expression, Expressing